[Evaluation of the indirect immunofluorescence test for the diagnosis of bancroftian filariasis using microfilariae W. bancrofti as the antigen, in Recife-PE, Brazil].
Identifieur interne : 012701 ( Main/Exploration ); précédent : 012700; suivant : 012702[Evaluation of the indirect immunofluorescence test for the diagnosis of bancroftian filariasis using microfilariae W. bancrofti as the antigen, in Recife-PE, Brazil].
Auteurs : G. Dreyer [Brésil] ; L. Andrade ; M. Espírito Santo ; Z. Medeiros ; I. Moura ; J. Ten Rio ; A. Rocha ; M I Casimiro ; E. Galdino ; E. DreyerSource :
- Revista do Instituto de Medicina Tropical de Sao Paulo [ 0036-4665 ]
Descripteurs français
- KwdFr :
- MESH :
- analyse : Antigènes d'helminthe.
- diagnostic : Filariose lymphatique.
- immunologie : Wuchereria bancrofti.
- Adolescent, Adulte, Adulte d'âge moyen, Animaux, Brésil, Femelle, Humains, Mâle, Sensibilité et spécificité, Soins ambulatoires, Sujet âgé, Technique d'immunofluorescence, Test ELISA.
- Wicri :
- geographic : Brésil.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antigens, Helminth.
- geographic : Brazil.
- diagnosis : Elephantiasis, Filarial.
- immunology : Wuchereria bancrofti.
- Adolescent, Adult, Aged, Ambulatory Care, Animals, Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Humans, Male, Middle Aged, Sensitivity and Specificity.
Abstract
The authors analysed the indirect immunofluorescence assay, for the diagnosis of bancroftian filariasis using papain treated W. bancrofti microfilariae as antigen, widely used in Recife-Brazil. Sera from 50 patients with several clinical forms of the disease including asymptomatic carriers, tropical pulmonary eosinophilia, elephantiasis, filarial fever and chyluria were analysed. For the control group, 50 individuals were selected, living at least 5 years in endemic area, with neither previous DEC treatment nor clinical-laboratory evidences of the disease, called normals endemic. The sensitivity and specificity were analysed taking into account different cut off values. It was not possible to differentiate infected individuals from the control group. It was not even possible to establish any correlation with IMF titers among different clinical presentation of the disease. Crossed reactions with various intestinal helminths were considered, but no relationship was found.
PubMed: 1844968
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">The authors analysed the indirect immunofluorescence assay, for the diagnosis of bancroftian filariasis using papain treated W. bancrofti microfilariae as antigen, widely used in Recife-Brazil. Sera from 50 patients with several clinical forms of the disease including asymptomatic carriers, tropical pulmonary eosinophilia, elephantiasis, filarial fever and chyluria were analysed. For the control group, 50 individuals were selected, living at least 5 years in endemic area, with neither previous DEC treatment nor clinical-laboratory evidences of the disease, called normals endemic. The sensitivity and specificity were analysed taking into account different cut off values. It was not possible to differentiate infected individuals from the control group. It was not even possible to establish any correlation with IMF titers among different clinical presentation of the disease. Crossed reactions with various intestinal helminths were considered, but no relationship was found.</div>
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